[ Pobierz całość w formacie PDF ]

Li, H., A. F. Oberhauser, S. B. Fowler, J. Clarke, and J. M. Fernandez.
Methods in Molecular Biology, Vol. 57: In Vitro Mutagenesis Protocols.
2000b. Atomic force microscopy reveals the mechanical design of a
M.K. Trower editor. Humana Press Inc., Totowa, NJ. 203 215.
modular protein. Proc. Natl. Acad. Sci. U. S. A. 97:6527 6531.
Bell, G. I. 1978. Models for the specific adhesion of cells to cells. Science. Lu, H., B. Isralewitz, A. Krammer, V. Vogel, and K. Schulten. 1998.
200:618 627. Unfolding of titin immunoglobulin domains by steered molecular dy-
namics simulation. Biophys. J. 75:662 671.
Best, R. B., L. Bin, A. Steward, V. Daggett, and J. Clarke. 2001. Can
non-mechanical proteins withstand force: stretching barnase by atomic Lu, H., and K. Schulten. 1999. Steered molecular dynamics simulations of
force microscopy and molecular dynamics simulation. Biophys. J. 81: force-induced protein domain unfolding. Proteins Struct. Funct. Genet.
2344 2356. 35:453 463.
Lu, H., and K. Schulten. 2000. The key event in force-induced unfolding
Binnig, G., C. F. Quate, and C. Gerber. 1986. Atomic force microscope.
of titin s immunoglobulin domains. Biophys. J. 79:51 65.
Phys. Rev. Lett. 56:930 933.
Makarov, D. E., P. K. Hansma, and H. Metiu. 2001. Kinetic Monte Carlo
Burnham, N. A., and R. J. Colton. 1989. Measuring the nanomechanical
properties and surface forces of materials using an atomic force micro- simulation of titin unfolding. J. Chem. Phys. 114:9663 9673.
scope. J. Vac. Sci. Technol. A7:2906 2913.
Marszalek, P. E., H. Lu, H. Li, M. Carrion-Vazquez, A. F. Oberhauser, K.
Schulten, and J. M. Fernandez. 1999. Mechanical unfolding intermedi-
Carrion-Vazquez, M., P. E. Marszalek, A. F. Oberhauser, and J. M.
ates in titin modules. Nature. 402:100  103.
Fernandez. 1999b. Atomic force microscopy captures length phenotypes
in single proteins. Proc. Natl. Acad. Sci. U. S. A. 96:11288 11292.
Merkel, R., P. Nassoy, A. Leung, K. Ritchie, and E. Evans. 1999. Energy
landscapes of receptor-ligand bonds explored with dynamic force spec-
Carrion-Vazquez, M., A. F. Oberhauser, T. E. Fisher, P. E. Marszalek, H.
troscopy. Nature. 397:50  53.
Li, and J. M. Fernandez. 2000. Mechanical design of proteins studied by
single-molecule force spectroscopy and protein engineering. Prog. Bio- Merritt, E. A., and M. E. P. Murphy. 1994. Raster3D Version 2.0- a
phys. Mol. Biol. 74:63 91. program for photorealistic molecular graphics. Acta Crystallogr. D.
50:869  873.
Carrion-Vazquez, M., A. F. Oberhauser, S. B. Fowler, P. E. Marszalek,
S. E. Broedel, J. Clarke, and J. M. Fernandez. 1999a. Mechanical and Mitsui, K., M. Hara, and A. Ikai. 1996. Mechanical unfolding of 2 mac-
chemical unfolding of a single protein: a comparison. Proc. Natl. Acad. roglobulin molecules with atomic force microscope. FEBS Lett. 385:
Sci. U. S. A. 96:3694 3699. 29 33.
Biophysical Journal 83(1) 458  472
472 Brockwell et al.
Myers, J. K., C. N. Pace, and J. M. Scholtz. 1995. Denaturant m values and Rief, M., F. Oesterhelt, B. Heymann, and H. E. Gaub. 1997a. Single
heat capacity changes: relation to changes in accessible surface areas of molecule force spectroscopy on polysaccharides by atomic force spec-
protein unfolding. Protein Sci. 4:2138 2148. troscopy. Science. 275:1295 1297.
Rief, M., J. Pascual, M. Saraste, and H. E. Gaub. 1999. Single molecule
Oberdörfer, Y., H. Fuchs, and A. Janshoff. 2000. Conformational analysis
force spectroscopy of spectrin repeats: low unfolding forces in helix
of native fibronectin by means of force spectroscopy. Langmuir. 16:
bundles. J. Mol. Biol. 286:553 561.
9955 9958.
Santoro, M., and D. W. Bolen. 1988. Unfolding free energy changes
Oberhauser, A. F., P. E. Marszalek, H. P. Erickson, and J. M. Fernandez.
determined by the linear extrapolation method: 1. Unfolding of phenyl-
1998. The molecular elasticity of the extracellular matrix protein tena-
methanesulfonyl -chymotrypsin using different denaturants. Biochem-
scin. Nature. 393:181 185.
istry. 27:8063 8068.
Oesterhelt, F., D. Oesterhelt, M. Pfeiffer, A. Engel, H. E. Gaub, and D. J. Smith, B. L., T. E. Schäffer, M. Viani, J. B. Thompson, N. A. Frederick,
Müller. 2000. Unfolding pathways of individual bacteriorhodopsins. J. Kindt, A. Belcher, G. D. Stucky, D. E. Morse, and P. K. Hansma.
Science. 288:143 146. 1999. Molecular mechanistic origin of the origin of the toughness of
natural adhesives, fibres and composites. Nature. 399:761 763.
Paci, E., and M. Karplus. 2000. Unfolding proteins by external forces and
Trinick, J. 1996. Cytoskeleton: titin as a scaffold and spring. Curr. Biol.
temperature: the importance of topology and energetics. Proc. Natl.
6:258  260.
Acad. Sci. U. S. A. 97:6521 6526.
Tskhovrebova, L., J. Trinick, J. A. Sleep, and R. M. Simmons. 1997.
Rief, M., J. M. Fernandez, and H. E. Gaub. 1998. Elastically coupled
Elasticity and unfolding of single molecules of the giant muscle protein
two-level systems as a model for biopolymer extensibility. Phys. Rev.
titin. Nature. 387:308  312.
Lett. 81:4764 4767.
Yang, G., C. Cecconi, W. A. Baase, I. R. Vetter, W. A. Breyer, J. A. Haack,
Rief, M., M. Gautel, F. Oesterhelt, J. M. Fernandez, and H. E. Gaub. B. W. Matthews, F. W. Dahlquist, and C. Bustamante. 2000. Solid-state
1997b. Reversible unfolding of individual titin immunoglobulin do- synthesis and mechanical unfolding of polymers of T4 lysozyme. Proc.
mains by AFM. Science. 276:1109 1111. Natl. Acad. Sci. U. S. A. 97:139 144.
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